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Western blot analysis of EECs treated with Tellimagrandin II: ( a ) protein immunoblot; ( b ) ALDH7A1; ( c ) <t>SMOX;</t> ( d ) NLRP3; ( e ) Caspase-1; ( f ) β-catenin; ( g ) cleaved Caspase-3; ( h ) BAX; ( i ) MMP2; ( j ) TIMP1. Each experiment was repeated three times. *:P <0.05; **:P <0.01; ***:P <0.001; ****P <0.0001.
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Western blot analysis of EECs treated with Tellimagrandin II: ( a ) protein immunoblot; ( b ) ALDH7A1; ( c ) <t>SMOX;</t> ( d ) NLRP3; ( e ) Caspase-1; ( f ) β-catenin; ( g ) cleaved Caspase-3; ( h ) BAX; ( i ) MMP2; ( j ) TIMP1. Each experiment was repeated three times. *:P <0.05; **:P <0.01; ***:P <0.001; ****P <0.0001.
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Image Search Results


Western blot analysis of EECs treated with Tellimagrandin II: ( a ) protein immunoblot; ( b ) ALDH7A1; ( c ) SMOX; ( d ) NLRP3; ( e ) Caspase-1; ( f ) β-catenin; ( g ) cleaved Caspase-3; ( h ) BAX; ( i ) MMP2; ( j ) TIMP1. Each experiment was repeated three times. *:P <0.05; **:P <0.01; ***:P <0.001; ****P <0.0001.

Journal: Journal of Inflammation Research

Article Title: Tellimagrandin II Stimulates Inflammasomes by Causing an Accumulation of 3-Aminopropanal, Which Promotes Apoptosis of Endometriotic Cells While Inhibiting Invasion

doi: 10.2147/JIR.S558146

Figure Lengend Snippet: Western blot analysis of EECs treated with Tellimagrandin II: ( a ) protein immunoblot; ( b ) ALDH7A1; ( c ) SMOX; ( d ) NLRP3; ( e ) Caspase-1; ( f ) β-catenin; ( g ) cleaved Caspase-3; ( h ) BAX; ( i ) MMP2; ( j ) TIMP1. Each experiment was repeated three times. *:P <0.05; **:P <0.01; ***:P <0.001; ****P <0.0001.

Article Snippet: Tellimagrandin II (Yongjian: B02179 ), Spermine Oxidase (SMOX) Polyclonal antibody (proteintech: 15052-1-AP), Aldehyde Dehydrogenase 7A1 (ALDH7A1) Polyclonal antibody (proteintech: 10368-1-AP), Anti-NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) Rabbit pAb (Servicebio: GB114320-50), Recombinant Anti-beta Catenin (β-Catenin) antibody (Servicebio: GB150016-100), Anti-Matrix Metallopeptidase 2 (MMP2) Rabbit pAb (Servicebio: GB11130-100), Anti- Tissue Inhibitors of Metalloproteinase 1 (TIMP1) Rabbit pAb (proteintech: 16644-1-AP), Anti-Caspase-3 Rabbit pAb (Servicebio: GB115600-100), Anti-Bcl-2-associated X(Bax) Rabbit pAb (Servicebio: GB11690-100), Anti-Caspase-1 Rabbit pAb (Servicebio: GB11383-100), DMEM/F12 (Glutamine and HEPES)(M&C GENE: CM10092), Fetal Bovine Serum Gold (YEASEN: 40130ES76), penicillin streptomycin mixture (Servicebio: P1400), 1×PBS buffer (Solarbio: P1020), trypsin-EDTA digestion solution (Phenol Red)(Solarbio: T1320), CCK-8 kit (Sola rbio: CA1210), Annexin V-FITC Apoptosis detection kit (Beyotime: C1062S), 96-well cell culture plate (Servicebio:) CCP-96H, SWE matrix adhesive (Solarbio: G4131-5ML), crystal violet staining solution (Solarbio: G1014-50ML), paraformaldehyde fixative (Solarbio: G1101-500ML), Transwell chamber (Corning: WG3422) Cell culture flasks (Corning: 430639), 12Z EECs line (Bioharbor: LH-H337), DMSO (Servicebio: GC203006-10mL), Propidium Iodide (proteintech: CM18819), Flow cytometer (BD: FACSAria II), Gel imaging system (Alpha: 2200) Electrophoresis apparatus (Thermo: EC250-90), Thermo Corporation, USA; Pendulum type decolorizing shaker (model: DS-2S100), Nanodrop micro spectrophotometer (Illumina: Nanodrop2000), sequencing platform machine (Illumina: NovaSeq X Plus).

Techniques: Western Blot

Molecular mechanism of Tellimagrandin II in the treatment of endometriosis. Tellimagrandin II (TII) may increase SMOX expression and decrease ALDH7 expression, leading to the accumulation of 3-aminopropanal in endometrial epithelial cells (EECs). The accumulated 3-aminopropanal can activate the inflammasome. Upon inflammasome activation, it may promote MMP2 expression via β-catenin and induce apoptosis by activating Caspase-1. However, whether TII inhibits invasion and promotes apoptosis in EECs through the inflammasome pathway requires further experimental verification.

Journal: Journal of Inflammation Research

Article Title: Tellimagrandin II Stimulates Inflammasomes by Causing an Accumulation of 3-Aminopropanal, Which Promotes Apoptosis of Endometriotic Cells While Inhibiting Invasion

doi: 10.2147/JIR.S558146

Figure Lengend Snippet: Molecular mechanism of Tellimagrandin II in the treatment of endometriosis. Tellimagrandin II (TII) may increase SMOX expression and decrease ALDH7 expression, leading to the accumulation of 3-aminopropanal in endometrial epithelial cells (EECs). The accumulated 3-aminopropanal can activate the inflammasome. Upon inflammasome activation, it may promote MMP2 expression via β-catenin and induce apoptosis by activating Caspase-1. However, whether TII inhibits invasion and promotes apoptosis in EECs through the inflammasome pathway requires further experimental verification.

Article Snippet: Tellimagrandin II (Yongjian: B02179 ), Spermine Oxidase (SMOX) Polyclonal antibody (proteintech: 15052-1-AP), Aldehyde Dehydrogenase 7A1 (ALDH7A1) Polyclonal antibody (proteintech: 10368-1-AP), Anti-NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) Rabbit pAb (Servicebio: GB114320-50), Recombinant Anti-beta Catenin (β-Catenin) antibody (Servicebio: GB150016-100), Anti-Matrix Metallopeptidase 2 (MMP2) Rabbit pAb (Servicebio: GB11130-100), Anti- Tissue Inhibitors of Metalloproteinase 1 (TIMP1) Rabbit pAb (proteintech: 16644-1-AP), Anti-Caspase-3 Rabbit pAb (Servicebio: GB115600-100), Anti-Bcl-2-associated X(Bax) Rabbit pAb (Servicebio: GB11690-100), Anti-Caspase-1 Rabbit pAb (Servicebio: GB11383-100), DMEM/F12 (Glutamine and HEPES)(M&C GENE: CM10092), Fetal Bovine Serum Gold (YEASEN: 40130ES76), penicillin streptomycin mixture (Servicebio: P1400), 1×PBS buffer (Solarbio: P1020), trypsin-EDTA digestion solution (Phenol Red)(Solarbio: T1320), CCK-8 kit (Sola rbio: CA1210), Annexin V-FITC Apoptosis detection kit (Beyotime: C1062S), 96-well cell culture plate (Servicebio:) CCP-96H, SWE matrix adhesive (Solarbio: G4131-5ML), crystal violet staining solution (Solarbio: G1014-50ML), paraformaldehyde fixative (Solarbio: G1101-500ML), Transwell chamber (Corning: WG3422) Cell culture flasks (Corning: 430639), 12Z EECs line (Bioharbor: LH-H337), DMSO (Servicebio: GC203006-10mL), Propidium Iodide (proteintech: CM18819), Flow cytometer (BD: FACSAria II), Gel imaging system (Alpha: 2200) Electrophoresis apparatus (Thermo: EC250-90), Thermo Corporation, USA; Pendulum type decolorizing shaker (model: DS-2S100), Nanodrop micro spectrophotometer (Illumina: Nanodrop2000), sequencing platform machine (Illumina: NovaSeq X Plus).

Techniques: Expressing, Activation Assay

Molecular docking diagram: ( a ) 3D representation of the docking between ALDH7A1 and Tellimagrandin II; ( b ) 2D representation of the docking between ALDH7A1 and Tellimagrandin II; ( c ) 3D representation of the docking between SMOX and Tellimagrandin II; ( d ) 2D representation of the docking between SMOX and Tellimagrandin II.

Journal: Journal of Inflammation Research

Article Title: Tellimagrandin II Stimulates Inflammasomes by Causing an Accumulation of 3-Aminopropanal, Which Promotes Apoptosis of Endometriotic Cells While Inhibiting Invasion

doi: 10.2147/JIR.S558146

Figure Lengend Snippet: Molecular docking diagram: ( a ) 3D representation of the docking between ALDH7A1 and Tellimagrandin II; ( b ) 2D representation of the docking between ALDH7A1 and Tellimagrandin II; ( c ) 3D representation of the docking between SMOX and Tellimagrandin II; ( d ) 2D representation of the docking between SMOX and Tellimagrandin II.

Article Snippet: Tellimagrandin II (Yongjian: B02179 ), Spermine Oxidase (SMOX) Polyclonal antibody (proteintech: 15052-1-AP), Aldehyde Dehydrogenase 7A1 (ALDH7A1) Polyclonal antibody (proteintech: 10368-1-AP), Anti-NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) Rabbit pAb (Servicebio: GB114320-50), Recombinant Anti-beta Catenin (β-Catenin) antibody (Servicebio: GB150016-100), Anti-Matrix Metallopeptidase 2 (MMP2) Rabbit pAb (Servicebio: GB11130-100), Anti- Tissue Inhibitors of Metalloproteinase 1 (TIMP1) Rabbit pAb (proteintech: 16644-1-AP), Anti-Caspase-3 Rabbit pAb (Servicebio: GB115600-100), Anti-Bcl-2-associated X(Bax) Rabbit pAb (Servicebio: GB11690-100), Anti-Caspase-1 Rabbit pAb (Servicebio: GB11383-100), DMEM/F12 (Glutamine and HEPES)(M&C GENE: CM10092), Fetal Bovine Serum Gold (YEASEN: 40130ES76), penicillin streptomycin mixture (Servicebio: P1400), 1×PBS buffer (Solarbio: P1020), trypsin-EDTA digestion solution (Phenol Red)(Solarbio: T1320), CCK-8 kit (Sola rbio: CA1210), Annexin V-FITC Apoptosis detection kit (Beyotime: C1062S), 96-well cell culture plate (Servicebio:) CCP-96H, SWE matrix adhesive (Solarbio: G4131-5ML), crystal violet staining solution (Solarbio: G1014-50ML), paraformaldehyde fixative (Solarbio: G1101-500ML), Transwell chamber (Corning: WG3422) Cell culture flasks (Corning: 430639), 12Z EECs line (Bioharbor: LH-H337), DMSO (Servicebio: GC203006-10mL), Propidium Iodide (proteintech: CM18819), Flow cytometer (BD: FACSAria II), Gel imaging system (Alpha: 2200) Electrophoresis apparatus (Thermo: EC250-90), Thermo Corporation, USA; Pendulum type decolorizing shaker (model: DS-2S100), Nanodrop micro spectrophotometer (Illumina: Nanodrop2000), sequencing platform machine (Illumina: NovaSeq X Plus).

Techniques:

qPCR primer sequence

Journal: International Journal of Medical Sciences

Article Title: Assessing the Role of Polyamine Metabolites in Blood and the DNA Methylation of Mycobacterium Tuberculosis in Patients with Multidrug-Resistant Tuberculosis

doi: 10.7150/ijms.102568

Figure Lengend Snippet: qPCR primer sequence

Article Snippet: PBMCs isolation medium was purchased from Tianjin Haoyang biological manufacture Co., Ltd. Spermine oxidase (SMOX) antibody, ornithine decarboxylase 1(ODC1) antibody, spermidine synthase (SRM) antibody and GAPDH antibody were purchased from Proteintech Group.

Techniques: Sequencing

Analysis of the levels of peripheral blood spermidine, spermine and polyamine metabolism-related enzymes in the HV and TB patients. ( A ) and ( B ) Concentrations of SPD and SPM by using HPLC in peripheral blood. ( C ) Correlation analysis between SPD and SPM levels in the HV. ( D ) Correlation analysis between SPD and SPM levels in TB patients. ( E ) The protein production levels of SMOX, ODC1 and SRM in HV and TB patients were detected by western blot. ( F ) Representative semi-quantification of protein band densities normalized to GAPDH from the western blot analysis. ** P < 0.01, and *** P < 0.0001.

Journal: International Journal of Medical Sciences

Article Title: Assessing the Role of Polyamine Metabolites in Blood and the DNA Methylation of Mycobacterium Tuberculosis in Patients with Multidrug-Resistant Tuberculosis

doi: 10.7150/ijms.102568

Figure Lengend Snippet: Analysis of the levels of peripheral blood spermidine, spermine and polyamine metabolism-related enzymes in the HV and TB patients. ( A ) and ( B ) Concentrations of SPD and SPM by using HPLC in peripheral blood. ( C ) Correlation analysis between SPD and SPM levels in the HV. ( D ) Correlation analysis between SPD and SPM levels in TB patients. ( E ) The protein production levels of SMOX, ODC1 and SRM in HV and TB patients were detected by western blot. ( F ) Representative semi-quantification of protein band densities normalized to GAPDH from the western blot analysis. ** P < 0.01, and *** P < 0.0001.

Article Snippet: PBMCs isolation medium was purchased from Tianjin Haoyang biological manufacture Co., Ltd. Spermine oxidase (SMOX) antibody, ornithine decarboxylase 1(ODC1) antibody, spermidine synthase (SRM) antibody and GAPDH antibody were purchased from Proteintech Group.

Techniques: Western Blot

Analysis of the levels of spermidine and spermine and related metabolic enzymes in the peripheral blood of the DS-TB patient group and the MDR-TB patient group. ( A-B ) Concentrations of SPD and SPM by HPLC in peripheral blood. ( C ) Correlation analysis between SPD and SPM levels in the DS-TB patient group. ( D ) Correlation analysis between SPD and SPM levels in MDR-TB patients. ( E ) Protein expression levels of SMOX, ODC1 and SRM by western blot. ( F ) Representative semi-quantification of protein band densities normalized to GAPDH from the western blot analysis. ( G - I ) The relative mRNA expression of SMOX, ODC1 and SRM by qPCR. ( J-L ) The levels of SSAT, HIF-1α and NOS in peripheral blood by ELISA. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Journal: International Journal of Medical Sciences

Article Title: Assessing the Role of Polyamine Metabolites in Blood and the DNA Methylation of Mycobacterium Tuberculosis in Patients with Multidrug-Resistant Tuberculosis

doi: 10.7150/ijms.102568

Figure Lengend Snippet: Analysis of the levels of spermidine and spermine and related metabolic enzymes in the peripheral blood of the DS-TB patient group and the MDR-TB patient group. ( A-B ) Concentrations of SPD and SPM by HPLC in peripheral blood. ( C ) Correlation analysis between SPD and SPM levels in the DS-TB patient group. ( D ) Correlation analysis between SPD and SPM levels in MDR-TB patients. ( E ) Protein expression levels of SMOX, ODC1 and SRM by western blot. ( F ) Representative semi-quantification of protein band densities normalized to GAPDH from the western blot analysis. ( G - I ) The relative mRNA expression of SMOX, ODC1 and SRM by qPCR. ( J-L ) The levels of SSAT, HIF-1α and NOS in peripheral blood by ELISA. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: PBMCs isolation medium was purchased from Tianjin Haoyang biological manufacture Co., Ltd. Spermine oxidase (SMOX) antibody, ornithine decarboxylase 1(ODC1) antibody, spermidine synthase (SRM) antibody and GAPDH antibody were purchased from Proteintech Group.

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay